Alexey Glukhov, Ph.D.
I am Assistant Professor at Department of Medicine, School of Medicine and Public Health, University of Wisconsin-Madison. My laboratory is a part of the Cellular and Molecular Arrhythmia Research Program (CMARP) involving multiple investigators working on a wide range of research projects exploring the molecular function of ion channels in human physiology, pharmacology and disease. I have a broad multidisciplinary background that combines my expertise in physiology, cell biology, biomedical engineering, biophysics, and confocal microscopy. I have an active, NIH- and AHA-funded, basic research laboratory focused on cardiovascular research which combines several state-of-the-art techniques, including high-resolution fluorescent optical mapping and scanning ion conductance microscopy equipped with super-resolution scanning patch-clamp. These are applied to investigate mechanisms involved in atrial, sinoatrial node, and ventricular cardiac remodeling and arrhythmogenesis following hypertension, heart failure, and atrial fibrillation among multiple levels of cardiac signaling: from protein expression, phosphorylation, and sub-cellular localization, protein-protein interaction, to propagation and repolarization of an intact heart. I am particularly interested in understanding the functionality of distinct subcellular microdomains in cardiac myocytes, such as transversal (t)-tubules, caveolae, and various costamers, and their functional role in the accumulation, neuro-hormonal and mechanical regulation of proteins responsible for normal and pathophysiological electrical activity and contraction of the heart, and how microdomain-targeted remodeling of those proteins contributes to abnormal cardiac electrophysiology and arrhythmogenesis associated with various pathologies.
Experience and Qualifications as Mentor: Since my faculty appointment at UW-Madison in 2015, I have mentored seven postdoctoral fellows, three graduate students, and twenty-five undergraduate students. Of those 35 trainees, 14 are women, and 2 are from groups that are traditionally underrepresented in science. I am committed to not only supporting and mentoring a diverse group of scientists, but also fostering an environment and climate within my group that is inclusive and supportive to all.
My trainees received multiple recognitions, including eight poster competition winning prizes, two Hilldale-Holstrom Undergraduate Student Fellowships, one undergraduate, one predoctoral and two postdoctoral AHA fellowships, one Goldwater Undergraduate Student Scholarship, one UW-Madison Cardiovascular Research Center Undergraduate Student Scholarship, two travel awards from the Biophysical Society, two AHA Career Development Awards, individual grants from UW-Madison Department of Medicine Research Committee, UW-Madison Institute of Translational and Clinical Research, and UW-Madison graduate programs.
I am a trainer on UW-Madison Cardiovascular Research Center NIH T32 program, Molecular and Cellular Pharmacology NIH T32 program, Cellular and Molecular Biology Graduate Program, UW Stem Cell and Regenerative Medicine Center Training Program, and on an AHA Predoctoral and Postdoctoral Research Fellowships, and serve on Molecular and Cellular Pharmacology NIH T32 Program Recruiting Committee from 2018.
Education and Training:
2012-2015 Postdoctoral training in Biophysics, Heart Science section at the National Heart & Lung Institute, Imperial College London, United Kingdom
2011-2012 Postdoctoral training in physiology, the Ohio State University, Columbus, USA
2007-2010 Postdoctoral training Biomedical Engineering, Washington University in St. Louis, USA
2007 Ph.D. in Biology, Specialty in Physiology, Institute of Theoretical and Experimental Biophysics of Russian Academy of Science and Cardiology Research Centre, Moscow, Russia
2003 M.Ss. in Biophysics, Moscow Institute of Physics and Technology, Russia
2001 B.S. in Biophysics, Moscow Institute of Physics and Technology, Russia
Complete list of publications (GoogleScholar)
Lab publications (from UW-Madison)
- Lang D, Medvedev R, Ratajczyk L, Zheng Z, Yuan X, Lim E, Han OY, Valdivia HH, Glukhov AV. Region-specific distribution of transversal-axial tubule system organization underlies heterogeneity of calcium dynamics in the right atria. Am J Physiol Heart Circ Physiol. 2022; 322(2):H269-H284.
- Turner D, Kang C, Mesirca P, Hong J, Mangoni ME, Glukhov AV, Sah R. Electrophysiological and Molecular Mechanisms of Sinoatrial Node Mechanosensitivity. Front Cardiovasc Med. 2021; 8:662410.
- Lang D, Glukhov AV. Cellular and molecular mechanisms of functional hierarchy of pacemaker clusters in the sinoatrial node: New insights into sick sinus syndrome. J Cardiovasc Dev Dis. 2021, 8(4), 43.
- Medvedev RY, Sanchez-Alonso JL, Mansfield CA, Judina A, Francis AJ, Pagiatakis C, Trayanova N, Glukhov AV, Miragoli M, Faggian G, Gorelik J. Local hyperactivation of L-type Ca2+ channels, increases spontaneous Ca2+ release activity and cellular hypertrophy in right ventricular myocytes from heart failure rats. Sci Rep. 2021; 11(1):4840.
- Tyan L, Turner D, Komp KR, Medvedev R, Lim E, Glukhov AV. Caveolin-3 is required for regulation of transient outward potassium current by angiotensin II in mouse atrial myocytes. Am J Physiol Heart Circ Physiol. 2021; 320(2): H787-H797.
- Egorov YV, Lang D, Tyan L, Turner D, Lim E, Piro ZD, Hernandez JJ, Lodin R, Wang R, Schmuck EG, Raval AN, Ralphe CJ, Kamp TJ, Rosenshtraukh LV, Glukhov AV. Caveolae-Mediated Activation of Mechanosensitive Chloride Channels Triggers Atrial Arrhythmogenesis. J Am Heart Assoc. 2019; 8(20): e012748.
- Lang D, Glukhov AV. Functional Microdomains in Heart’s Pacemaker: A Step Beyond Classical Electrophysiology and Remodeling. Front Physiol. 2018; 9:1686.
- Glukhov AV, Fedorov VV, Lou Q, Ravikumar VK, Kalish PW, Schuessler RB, Moazami N, Efimov IR. Transmural dispersion of repolarization in failing and nonfailing human ventricle. Circulation Research. 2010; 106(5), 981-91.
- Glukhov AV, Fedorov VV, Kalish PW, Ravikumar VK, Lou Q, Janks D, Schuessler RB, Moazami N, Efimov IR. Conduction remodeling in human end-stage nonischemic left ventricular cardiomyopathy. Circulation. 2012; 125(15), 1835-1847.
- Glukhov AV, Balycheva M, Sanchez-Alonso JL, Ilkan Z, Alvarez-Laviada A, Bhogal N, Diakonov I, Schobesberger S, Sikkel MB, Bhargava A, Faggian G, Punjabi PP, Houser SR, Gorelik J. Direct evidence for microdomain-specific localization and remodeling of functional L-type calcium channels in rat and human atrial myocytes. Circulation. 2015; 132(25):2372-84.
- Miragoli M, Glukhov AV. Atrial fibrillation and fibrosis: Beyond the cardiomyocyte centric view. BioMed Res Int. 2015; 798768.
- Glukhov AV, Fedorov VV, Mohler PJ, Anderson ME, Efimov IR. Functional anatomy of the murine sinus node: Evidence from high-resolution optical mapping. Am J Physiol Heart Circ Physiol. 2010; 299(2): H482-91.
- Glukhov AV, Hage LT, Hansen BJ, Pedraza-Toscano A, Vargas-Pinto P, Hamlin RL, Weiss R, Carnes CA, Billman GE, Fedorov VV. Sinoatrial node reentry in a canine chronic left ventricular infarct model: role of intranodal fibrosis and heterogeneity of refractoriness. Circ Arrhythm Electrophysiol. 2013; 6(5):984-94.
- Glukhov AV, Kalyanasundaram A, Lou Q, Hage LT, Hansen BJ, Belevych AE, Mohler PJ, Knollmann BC, Periasamy M, Györke S, Fedorov VV. Calsequestrin 2 deletion causes sinoatrial node dysfunction and atrial arrhythmias associated with altered sarcoplasmic reticulum calcium cycling and degenerative fibrosis within the mouse atrial pacemaker complex. European Heart Journal, 2015; 36(11), 686-97.
- Sharifov OF, Fedorov VV, Beloshapko GG, Glukhov AV, Iushmanova AV, Rozenshtraukh LV. Roles of adrenergic and cholinergic stimulation in spontaneous atrial fibrillation in dogs. Journal of American College of Cardiology, 2004; 43(3), 483-90.
- NIH/NHLBI R01 HL41214 (2018-2023), Glukhov (PI), “Functional Microdomains in the Heart’s Pacemaker: A New Dimension of Cardiac Remodeling”
- NIH/NHLBI 1R01HL139738-01A1 (2018-2022), Eckhardt (PI, Glukhov (Co-I), “KCNJ2 Mutation-Induced Arrhythmia Mechanisms in CPVT Phenotypes”
- NIH/NHLBI 1R01HL146652-01A1 (2020-2023), Zhao (PI), Glukhov (Co-I), “Engineered anisotropic and vascularized human cardiac patch”
- Office of the Vice Chancellor for Research and Graduate Education (OVCRGE)/Wisconsin Alumni Research Foundation (WARF) Fall Competition Award (2021-2022), Glukhov (PI), “Atrial-like Engineered Cardiac Tissue as a Novel Model for Cardiac Pressure Overload and Atrial Fibrillation Pathogenesis”